Chapter 13 Infographic: Illustration of a typical HRM methodology workflow. The initial substrate can be a single or multi-ingredient product from raw or processed plant material. Following DNA extraction, taxon-specific primers are developed based on DNA barcodes or other molecular markers and tested in silico. An intercalating fluorescent dye is added to the PCR reaction, which allows the detection of the PCR amplicons by the HRM equipment during the melting process. The output consists of melting graphs and a statistical report including Genotype Confidence Percentages (GCPs) allowing accurate discrimination between the reference and the analysed taxa.