"Experiment or sample considerations";"Recommended method(s)";Comments "Whole genome or organellar sequencing project (genome skimming, genome resequencing, de novo genome assembly)";"Illumina, PacBio, or a combination of both";"Illumina is the method of choice for resequencing for high throughput short read projects due to its high read accuracy" Barcoding;"Sanger sequencing or PacBio CCS";"Larger projects are moving to PacBio CCS to reduce costs. Multiplexing very large numbers of samples is necessary to optimise costs" "Metabarcoding/Target capture";"Illumina, MGI, DNBSEQ, or Ion Torrent";"PacBio and/or Nanopore may also be considered if the sequence is expected to be highly repetitive" "Heavily degraded samples (i.e., herbarium or ancient DNA samples)";"Illumina (or Ion Torrent)";"PacBio may also be relevant for the study of post-genetic modifications often found in ancient DNA samples, or if dealing with hard-to-phase sequences" "On-site sequencing";"Nanopore (MinION) Hi-C/3C-Seq/Capture-C (Illumina)";