"Proteins and RNA";"What compounds define the chemical composition of your samples?";"Polysaccharides (starch, sugars)";Polyphenolics;RNA;"(plant secondary metabolites like: tannins, flavonoids, terpenoids, etc.)" "Understand the specific properties of your samples for DNA extraction";"Can co-purify with DNA";"Can co-precipitate with DNA";"When bound to DNA very hard to remove in extraction" "dependending on the age of the samples and how they were conserved";"Results in a sticky viscous consistency to DNA pellet after centrifugation" "Inhibition of enzymes used for molecular techniques (restriction endonucleases, polymerases, and ligases (Pandey et al. 1996))";"Results in contaminated pellets not usable for many downstream analyses (John 1992; Peterson et al. 1997)" "Adherence to wells in agarose gel residing in long smears of bands detected in gel (Sharma et al. 2002)" "Consider applying mitigation strategies to overcome difficulties in extracting DNA from your samples";"RNA removable with DNase-free RNase A or ethanol precipitation using lithium chloride";"Removal via highly concentrated sodium chloride (NaCl) in extraction buffers leading to increased solubility in ethanol";"Binder compounds polyvinyl pyrrolidone (PVP) or polypyrrolidone (PVPP) can be used in extraction buffers to absorb polyphenols before polymerization with DNA" "Proteins can be removed by i) inclusion of detergents (cetyltrimethylammonium bromide (CTAB), SDS) in extraction buffer" "Combination of NaCl and cationic detergent CTAB" "CTAB with differential precipitation (Murray and Thompson 1980)" "Use of antioxidant compounds (BME, DDT, ascorbic acid, iso-ascorbate) in buffer to prevent polymerization (Pich and Schubert 1993; Puchooa 2004)" "ii) protein denaturants e.g., β-mercaptoethanol (BME), dithiothreitol (DTT)" "iii) enzymatic proteases e.g., proteinase K"