Plant DNA

Storing and preparing plant material for DNA extraction

DNA extraction protocols

DNA quantification and quality assessment

Approaches to challenging DNA extractions

Concluding remarks

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Introduction

Ethical and legal aspects

Sampling museum collections

Laboratory work with historical samples

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Introduction

Detection of DNA from aquatic environments

Experimental design

Conclusion and prospects

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Introduction

Soil DNA: degradation, persistence, and decay

Soil memory

Designing a soil eDNA study

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Background

Pollen DNA extraction

Molecular methods for pollen identification

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Why use DNA for the identification of food and medicine?

DNA-based methods to identify plants in food and medicine

DNA isolation from food and medicines

The reality of DNA-based identification

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Background

Experimental design

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Background

Experimental design

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Introduction

Sequencing platforms

Which sequencing platform?

Perspectives

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DNA barcoding

DNA barcoding and species delimitation

DNA barcoding for plants

Hands-on plant DNA barcoding

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Background

Setting up a metabarcoding study

Future of metabarcoding

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Background

The process of metagenomics

Future of metagenomics

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Introduction

High resolution melting analysis

HRM assay and reagent optimization

Advantages and limitations of HRM

HRM applications

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Introduction

What is target capture?

Use of target capture for molecular identification

Designing a target capture experiment

Concluding remarks

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Background

Experimental design

Bioinformatics

Applications of transcriptomics in species identification

Conclusion and future perspectives

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Introduction

Sample to sequence: an overview of the workflow for genome sequencing

Genome skimming

Genome resequencing

De novo whole genome sequencing

Considerations and example applications

Conclusion

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What is a species?

Essential considerations to implement a species delimitation study

DNA-based species delimitation methods

Allopolyploidization and its impact on species delimitation

Conclusions

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Introduction

Sequencing quality control

Database alignment

Alignment-free methods

Assembly or mapping for multisequence sample alignments

Other considerations

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Introduction

Data sources used in systematics

Advances in molecular phylogenetics

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Museomics in plant research

Plant material in museum collections

Museum collections: fundamental and applied research

Ethical museomics, fair and equitable sharing, and co-design of research

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Introduction

Applications

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Plants as medicine

Quality control and authentication of herbal medicines

DNA-based identification of adulterants in herbal medicines

DNA-based identification in ethnopharmacology

DNA-based identification in natural product research and bioprospecting

Legal framework

Future perspectives of DNA-based identification for healthcare

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Introduction

Food hazards and impacts

Authentication of plant-based food stuffs

From farm to fork - traceability

Methodological approaches for the detection of plant food hazards

Conclusions and future prospects

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Introduction

Assessing plant DNA from the environment: power, precautions, and limitations

Substrates for eDNA in environmental and biodiversity assessments

Beyond eDNA samples: assessing biodiversity through eDNA biotic samplers

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Introduction

Methods for identification of plants in trade

Recommendations to improve wildlife trade monitoring

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Introduction

DNA technology for forensic plant analysis

Use of palynology in forensics

Forensic case studies

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